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Antibodies sure to the blocking peptide no more bind towards the epitope over the focus on protein. By comparing the staining from the blocked antibody compared to the antibody by yourself, you can see which staining is certain.
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This guidebook outlines even further possible optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
This tutorial outlines additional feasible optimization of cellular and immunoassay parameters to be certain the best possible outcomes are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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Blocking peptides are peptides that bind precisely towards the target antibody and block antibody binding. These peptide usually contains the epitope regarded via the antibody.
Target Details Cyclin E belongs on the hugely conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.
Overexpression of this gene has long been observed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was observed to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in selling mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants were claimed but specific nucleotide sequence data isn't nevertheless out there.
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